The University of Arizona

Setting up a lab to perform animal activities

Report Misconduct

University Ethics and Compliance Hotline:


Allergy Alert Sign: Print and place on the outside of the doors to areas where animals are used. This sign is used to alert non-animal users to the potential for allergens and to provide information on how to get a respirator, if desired.

Emergency Contact Sign: Print, complete and place on the inside of the doors to areas where animals are used. This sign is used in the event that there is an emergency and first responders find animals in the lab. It also provides contact information for animal welfare issues, as required by regulation.

Animal Hazards Checklist: Select the applicable form for your location, print, complete and file as appropriate. This is part of the Occupational Health Program associated with animal use. The form is a prompt to look for hazards and to deal with them in a timely manner. On the yearly anniversary of your protocol, you will be asked to complete a Continuing Review. One of the questions will be a prompt to review the Animal Hazards Checklist.

Survival Surgery and Post-Operative Monitoring Form/Non-Survival Surgery Form: Sample surgical records are available for both survival and non-survival surgery. Feel free to use these forms or adapt them to what works for you. These are the records that the IACUC review during the semi-annual inspections. Note that surgical records must be accessible during IACUC inspections. We suggest using a binder to keep all records centrally and making copies for individual lab notebooks.

Non-Surgical Analgesia Record: This is a sample non-surgical analgesia record. Same comments apply for this form as for the Surgery and Post-Operative Monitoring Form/Non-Survival Surgery Form above.

Drugs: We look at storage, grade and expiration date of clinical drugs, e.g., isoflurane, ketamine, xylazine, ophthalmic ointment, and surgical glue. These types of drugs should be pharmaceutical grade and not expired. A description of pharmaceutical grade for the IACUC purposes is found in IACUC Policy 210.

Ketamine and ketamine cocktails should be stored as for controlled substances (for more information contact Research Laboratory & Safety Services). The IACUC does not inspect the DEA paperwork associated with controlled substances, but we are required to ensure that they are stored appropriately. Anesthetic cocktails must be stored and labelled as described in IACUC Policy 210.

Surgical location: This should be away from heavy traffic areas, if at all possible. There should also be a physically separate area for preparation of the animal (hair removal, surgical scrub). This can be on the same bench, but it must be a distinct area from the surgical location.

CO2 euthanasia:  IACUC Guidance 214 describes the type of chamber, CO2source, flow meter, and method for confirming death that meet the regulations.

Loss Compensation: Loss Compensation Insurance Claim Form Application for the loss of animals due to infectious disease or equipment failure.